Non-structural protein 1 of H3N2 influenza A virus induces nucleolar stress via interaction with nucleolin.
Identifieur interne : 000A79 ( Main/Exploration ); précédent : 000A78; suivant : 000A80Non-structural protein 1 of H3N2 influenza A virus induces nucleolar stress via interaction with nucleolin.
Auteurs : Yinxia Yan [République populaire de Chine] ; Yongming Du [République populaire de Chine] ; Gefei Wang [République populaire de Chine] ; Kangsheng Li [République populaire de Chine]Source :
- Scientific reports [ 2045-2322 ] ; 2017.
Descripteurs français
- KwdFr :
- ARN ribosomique (génétique), Animaux, Cellules A549, Cellules rénales canines Madin-Darby, Chiens, Grippe humaine (génétique), Grippe humaine (virologie), Humains, Infections à Orthomyxoviridae (génétique), Infections à Orthomyxoviridae (virologie), Liaison aux protéines (génétique), Lignée cellulaire, Lignée cellulaire tumorale, Maladies des chiens (génétique), Maladies des chiens (virologie), Nucléole (génétique), Nucléole (virologie), Phosphoprotéines (génétique), Protéines de liaison à l'ARN (génétique), Protéines virales non structurales (génétique), RNA polymerase I (génétique), Régions promotrices (génétique) (génétique), Sous-type H3N2 du virus de la grippe A (génétique), Transcription génétique (génétique).
- MESH :
- génétique : ARN ribosomique, Grippe humaine, Infections à Orthomyxoviridae, Liaison aux protéines, Maladies des chiens, Nucléole, Phosphoprotéines, Protéines de liaison à l'ARN, Protéines virales non structurales, RNA polymerase I, Régions promotrices (génétique), Sous-type H3N2 du virus de la grippe A, Transcription génétique.
- virologie : Grippe humaine, Infections à Orthomyxoviridae, Maladies des chiens, Nucléole.
- Animaux, Cellules A549, Cellules rénales canines Madin-Darby, Chiens, Humains, Lignée cellulaire, Lignée cellulaire tumorale.
English descriptors
- KwdEn :
- A549 Cells, Animals, Cell Line, Cell Line, Tumor, Cell Nucleolus (genetics), Cell Nucleolus (virology), Dog Diseases (genetics), Dog Diseases (virology), Dogs, Humans, Influenza A Virus, H3N2 Subtype (genetics), Influenza, Human (genetics), Influenza, Human (virology), Madin Darby Canine Kidney Cells, Orthomyxoviridae Infections (genetics), Orthomyxoviridae Infections (virology), Phosphoproteins (genetics), Promoter Regions, Genetic (genetics), Protein Binding (genetics), RNA Polymerase I (genetics), RNA, Ribosomal (genetics), RNA-Binding Proteins (genetics), Transcription, Genetic (genetics), Viral Nonstructural Proteins (genetics).
- MESH :
- chemical , genetics : Phosphoproteins, RNA Polymerase I, RNA, Ribosomal, RNA-Binding Proteins, Viral Nonstructural Proteins.
- genetics : Cell Nucleolus, Dog Diseases, Influenza A Virus, H3N2 Subtype, Influenza, Human, Orthomyxoviridae Infections, Promoter Regions, Genetic, Protein Binding, Transcription, Genetic.
- virology : Cell Nucleolus, Dog Diseases, Influenza, Human, Orthomyxoviridae Infections.
- A549 Cells, Animals, Cell Line, Cell Line, Tumor, Dogs, Humans, Madin Darby Canine Kidney Cells.
Abstract
The nucleolus is a stress sensor associated with cell cycle progression and a central hub for the replication of pathogenic RNA viruses. However, the role of nucleolus in influenza A virus infection has not been well studied. Here we show that the interaction between NS1 protein of influenza A/Shantou/602/06 (H3N2) and nucleolin, a ubiquitous protein of nucleolus repressed RNA Pol I-dependent transcription via establishing hyper-methylation in the UCE of rRNA gene promoter. NS1 expressed cells showed significant association of ribosomal proteins with MDM2, and p53 accumulation, suggesting induced nucleolar stress. Disruption of the interaction of NS1 with nucleolin or overexpression of nucleolin in NS1 expressed cells revived RNA Pol I-dependent transcription, indicating nucleolin could be one target for NS1 to repress rRNA synthesis of host cells. Our present study suggests that NS1 protein of H3N2 could induce nucleolar stress based on epigenetic alteration of rRNA gene promoter via interaction with nucleolin.
DOI: 10.1038/s41598-017-18087-2
PubMed: 29259342
Affiliations:
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Le document en format XML
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sort="Wang, Gefei" uniqKey="Wang G" first="Gefei" last="Wang">Gefei Wang</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Infectious Diseases and Molecular Immunopathology of Guangdong Province, Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province, China. gefeiwang@stu.edu.cn.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Infectious Diseases and Molecular Immunopathology of Guangdong Province, Department of Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province</wicri:regionArea><wicri:noRegion>Guangdong Province</wicri:noRegion></affiliation></author><author><name sortKey="Li, Kangsheng" sort="Li, Kangsheng" uniqKey="Li K" first="Kangsheng" last="Li">Kangsheng Li</name><affiliation wicri:level="1"><nlm:affiliation>Key Laboratory of Infectious Diseases and Molecular Immunopathology of Guangdong Province, 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(genetics)</term><term>Dog Diseases (virology)</term><term>Dogs</term><term>Humans</term><term>Influenza A Virus, H3N2 Subtype (genetics)</term><term>Influenza, Human (genetics)</term><term>Influenza, Human (virology)</term><term>Madin Darby Canine Kidney Cells</term><term>Orthomyxoviridae Infections (genetics)</term><term>Orthomyxoviridae Infections (virology)</term><term>Phosphoproteins (genetics)</term><term>Promoter Regions, Genetic (genetics)</term><term>Protein Binding (genetics)</term><term>RNA Polymerase I (genetics)</term><term>RNA, Ribosomal (genetics)</term><term>RNA-Binding Proteins (genetics)</term><term>Transcription, Genetic (genetics)</term><term>Viral Nonstructural Proteins (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN ribosomique (génétique)</term><term>Animaux</term><term>Cellules A549</term><term>Cellules rénales canines Madin-Darby</term><term>Chiens</term><term>Grippe humaine (génétique)</term><term>Grippe humaine (virologie)</term><term>Humains</term><term>Infections à Orthomyxoviridae (génétique)</term><term>Infections à Orthomyxoviridae (virologie)</term><term>Liaison aux protéines (génétique)</term><term>Lignée cellulaire</term><term>Lignée cellulaire tumorale</term><term>Maladies des chiens (génétique)</term><term>Maladies des chiens (virologie)</term><term>Nucléole (génétique)</term><term>Nucléole (virologie)</term><term>Phosphoprotéines (génétique)</term><term>Protéines de liaison à l'ARN (génétique)</term><term>Protéines virales non structurales (génétique)</term><term>RNA polymerase I (génétique)</term><term>Régions promotrices (génétique) (génétique)</term><term>Sous-type H3N2 du virus de la grippe A (génétique)</term><term>Transcription génétique (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Phosphoproteins</term><term>RNA Polymerase I</term><term>RNA, Ribosomal</term><term>RNA-Binding Proteins</term><term>Viral Nonstructural Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Cell Nucleolus</term><term>Dog Diseases</term><term>Influenza A Virus, H3N2 Subtype</term><term>Influenza, Human</term><term>Orthomyxoviridae Infections</term><term>Promoter Regions, Genetic</term><term>Protein Binding</term><term>Transcription, Genetic</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN ribosomique</term><term>Grippe humaine</term><term>Infections à Orthomyxoviridae</term><term>Liaison aux protéines</term><term>Maladies des chiens</term><term>Nucléole</term><term>Phosphoprotéines</term><term>Protéines de liaison à l'ARN</term><term>Protéines virales non structurales</term><term>RNA polymerase I</term><term>Régions promotrices (génétique)</term><term>Sous-type H3N2 du virus de la grippe A</term><term>Transcription génétique</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Grippe humaine</term><term>Infections à Orthomyxoviridae</term><term>Maladies des chiens</term><term>Nucléole</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Nucleolus</term><term>Dog Diseases</term><term>Influenza, Human</term><term>Orthomyxoviridae Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>A549 Cells</term><term>Animals</term><term>Cell Line</term><term>Cell Line, Tumor</term><term>Dogs</term><term>Humans</term><term>Madin Darby Canine Kidney Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules A549</term><term>Cellules rénales canines Madin-Darby</term><term>Chiens</term><term>Humains</term><term>Lignée cellulaire</term><term>Lignée cellulaire tumorale</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The nucleolus is a stress sensor associated with cell cycle progression and a central hub for the replication of pathogenic RNA viruses. However, the role of nucleolus in influenza A virus infection has not been well studied. Here we show that the interaction between NS1 protein of influenza A/Shantou/602/06 (H3N2) and nucleolin, a ubiquitous protein of nucleolus repressed RNA Pol I-dependent transcription via establishing hyper-methylation in the UCE of rRNA gene promoter. NS1 expressed cells showed significant association of ribosomal proteins with MDM2, and p53 accumulation, suggesting induced nucleolar stress. Disruption of the interaction of NS1 with nucleolin or overexpression of nucleolin in NS1 expressed cells revived RNA Pol I-dependent transcription, indicating nucleolin could be one target for NS1 to repress rRNA synthesis of host cells. Our present study suggests that NS1 protein of H3N2 could induce nucleolar stress based on epigenetic alteration of rRNA gene promoter via interaction with nucleolin.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Yan, Yinxia" sort="Yan, Yinxia" uniqKey="Yan Y" first="Yinxia" last="Yan">Yinxia Yan</name></noRegion><name sortKey="Du, Yongming" sort="Du, Yongming" uniqKey="Du Y" first="Yongming" last="Du">Yongming Du</name><name sortKey="Li, Kangsheng" sort="Li, Kangsheng" uniqKey="Li K" first="Kangsheng" last="Li">Kangsheng Li</name><name sortKey="Wang, Gefei" sort="Wang, Gefei" uniqKey="Wang G" first="Gefei" last="Wang">Gefei Wang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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